منابع مشابه
Controlled Production of Proliferating Somatic Cell Hybrids
The techniques described permit the controlled production of large numbers of proliferating somatic cell hybrids in a relatively short period of time. Sendai virus is used to promote cell hybridization. beta-propriolactone is employed as the inactivating agent of Sendai virus since it produces complete loss of viral infectivity while preserving viral fusion capacity. Cells are fused in monolaye...
متن کاملKaryological properties of human-mouse somatic hybrids.
Karyological examinations performed 24 hr to 11 days after Sendai virus-induced fusion of mouse cells of the permanent line 3T3 and human diploid cells, suggest that hybrids with extensive loss of human chromosomes derive from fusion involving all the chromosomes of both parental cells. Examination of 39 independent clones isolated from two different crosses between L mouse cells and cells of t...
متن کاملIntegrative genomic analysis of human ribosomal DNA
The transcription of ribosomal RNA (rRNA) is critical to life. Despite its importance, ribosomal DNA (rDNA) is not included in current genome assemblies and, consequently, genomic analyses to date have excluded rDNA. Here, we show that short sequence reads can be aligned to a genome assembly containing a single rDNA repeat. Integrated analysis of ChIP-seq, DNase-seq, MNase-seq and RNA-seq data ...
متن کاملSomatic-cell hybrids producing antibodies against CEA.
Monoclonal antibodies to carcinoembryonic antigen (CEA) promise improved specificity for the measurement of this widely expressed human cancer antigen. A mouse monoclonal antibody binds weakly to CEA in perchloric acid extracts of tumour but strongly to CEA similarly isolated from serum, and its spectrum of cancer detection differs from conventional antisera.
متن کاملFate of mitochondrial DNA in human-mouse somatic cell hybrids (density gradient centrifugation-ethidium bromide-karyotype).
Several hybrid lines between human and mouse somatic cells, containing one or two complements of mouse chromosomes and a reduced complement of human chromosomes, have been examined for the presence of mouse and human mitochondrial DNAs. For this analysis, advantage was taken of the fact that these two types of mitochondrial DNA have a buoyant density difference in CsCl gradients of 0.008 g/cm(3...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant tissue culture letters
سال: 1984
ISSN: 1884-7706,0289-5773
DOI: 10.5511/plantbiotechnology1984.1.43